Multiplexed detection for biomolecules tagged to magnetic nanoparticles using a miniaturized AC magnetic susceptometer
نویسندگان
چکیده
A novel multiplexed sensing scheme based on Brownian relaxation for biomolecules tagged to magnetic nanoparticles (MNPs) in liquid environment is proposed. Feasibility of the technique has been verified by the experiments with the mixture of differently sized magnetic nanoparticles using a newly developed room temperature, miniaturized AC magnetic susceptometer. The AC magnetic susceptibility measurements of Brownian relaxation of MNPs verify the sensing modality that proves the resonant frequency of imaginary susceptibility is inversely proportional to effective hydrodynamic size of MNPs. The proposed Brownian sensing scheme has the potential for multiplexed analysis of multiple biological binding events on functionalized MNPs. The performances were verified for individual and mixtures of monodisperse iron oxide MNPs in solution with carboxylic acid group with core diameters of 15, 25, 35, 50nm using the proposed susceptometer. The approach is readily compatible with lab-on-chip applications in medical diagnostics, and can be used for affinity-based biosensing.
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